What is E-Polylysine?

E-Polylysine (ε-Polylysine) is a natural antimicrobial peptide used as a food preservative to inhibit the growth of Gram-positive and some Gram-negative bacteria, yeasts, and molds. It is widely applied in food, pharmaceuticals, and cosmetics for its broad-spectrum antimicrobial activity, biodegradability, and safety.

Approved by major regulatory bodies such as the FDA, EFSA, and WHO, E-Polylysine is GRAS (Generally Recognized as Safe) and listed under E-Number E239 in the European food additive system.

Epsilon-Polylysine is a homopolymer of Llysine, one of the essential amino acids. It’s connected by ε-amido bond compounded by a ε-amido of l-lysine and a a-carboxyl of another l-lysine. It is prepared from a fermentation for process using Streptomyces albulus under aerobic condition.

ε-Polylysine is commercially used as a food preservative in Japan, the republic of Korea and in the USA. Food products containing polylysine are mainly found in Japan. The use of polylysine is common in food applications like: boiled rice, cooked vegetables, soups, noodles and sliced fish (sushi).

---

E-Polylysine Product Details

Source of E-Polylysine

E-Polylysine is produced naturally by bacterial fermentation using Streptomyces albulus. This fermentation-based production ensures high purity and eco-friendly synthesis.

Chemical Information

• CAS Number: 28211-04-3
• Chemical Formula: (C6H12N2O)n
• Appearance: White to yellow powder, no caking and unusual odor.
• Solubility: Soluble in water, stable in acidic and neutral conditions
• Stability: 120°C 20min No activity loss,100°C 30min No activity loss,80°C 60min No activity loss, pH1–13 no activity loss, when pH<3 or pH>10 can not be existed for a long period
• Storage: In cool (0-20°C), dry conditions, away from direct sunlight, in original closed packages.

E-Polylysine Specifications

Parameter	Specification
Assay (E-Polylysine content)	≥94%
Solubility	Water-soluble
pH	4.0-6.5 (1% Aqueous Solution)
Loss on drying	≤5.0%
Heavy Metals	≤10 ppm
Lead (Pb)	≤1 ppm
Arsenic (As)	≤1 ppm

---

Key Advantages & Benefits of E-Polylysine

✅ Natural & Safe – Biodegradable, non-toxic, and environmentally friendly
✅ Broad-Spectrum Antimicrobial – Effective against Gram-positive and some Gram-negative bacteria, molds, and yeasts
✅ Enhances Food Safety – Prevents foodborne pathogens such as Listeria monocytogenes and E. coli
✅ Extends Shelf Life – Reduces spoilage and extends food freshness
✅ Heat & pH Stability – Retains antimicrobial activity even after high-temperature processing
✅ No Impact on Taste or Texture – Preserves the natural characteristics of food products
✅ Global Regulatory Approval – Approved by FDA, EFSA, WHO, and China GB2760

---

Functions of E-Polylysine

1. Epsilon-Polylysine can greatly inhibit the growth of bacteria, yeasts and mmolds bacteria, especially to the Gram- bacteria which can not easily inhibit by others.
2. As a good preservative, Epsilon-Polylysine is characterized as being high safety, water-soluble, stable at high temperatures, widely usage, non prejudice to the taste, antibacterial in neutral or subacidity conditions.
3.  Because of the stability at high temperatures, it can inhibit some heat-resistant bacillus after heat treatment and some tadpole-form noncontractile siphovirus as well.
4. Epsilon-Polylysine can stop small intestine fat absorption by tbyinhibiting pancreatic lipase activities, so it can be the diet therapy for obese patients.
5. Besides, Epsilon-Polylysine also can be used in cosmetics, disinfectantsor cleaners for food machinery or hutch, etc.

• Food Preservation – Protects food products from microbial contamination
• Bacterial Control in Pharmaceuticals – Used in topical applications and healthcare formulations
• Biofilm Prevention – Reduces bacterial adhesion in food processing and industrial applications
• Eco-Friendly Alternative to Chemical Preservatives – Helps manufacturers meet clean-label demands

---

Applications of E-Polylysine

Epsilon-Polylysine is to serve as antimicrobial agent or preservatives. It is used to food, cosmetics, personal care, disinfection products by preventing growth of microoganisms.

1. Food Industry

• Dairy Products – Cheese, yogurt, and milk-based products to prevent spoilage
• Meat & Poultry – Processed meats, sausages, and seafood to inhibit bacterial growth
• Beverages – Juices, teas, and fermented drinks for microbial control
• Bakery & Confectionery – Cakes, pastries, and snacks to extend shelf life
• Plant-Based Foods – Used in vegan and organic food formulations

2. Pharmaceutical & Healthcare

• Oral Care – Incorporated into toothpaste and mouthwash for antibacterial benefits
• Wound Care – Applied in bandages and medical dressings to prevent infections

3. Cosmetics & Personal Care

• Skin Care – Used in lotions, creams, and serums for microbial stability
• Hair Care – Added to shampoos and conditioners to prevent microbial contamination

---

Production Process of E-Polylysine

1️⃣ Raw Materials Preparation – Glucose, yeast powder, potassium dihydrogen phosphate, ammonium sulfate
2️⃣ Culture Medium Preparation
3️⃣ Seed Cultivation

• First-level seed pot
• Second-level seed pot
  4️⃣ Fermentation Process – Bacteria ferment in a controlled environment (pH, dissolved oxygen, nutrition, foam)
  5️⃣ Fermentation Broth Collection
  6️⃣ Filtration – Plate and frame filter to remove bacteria residue
  7️⃣ Purification
• Ion exchange
• Bleaching (with activated carbon)
• Ceramic filtration & nanofiltration
  8️⃣ Concentration
  9️⃣ Spray Drying
  🔟 Final Product: E-Polylysine Powder

This structured process ensures high-purity E-Polylysine with excellent antimicrobial properties for food, pharmaceuticals, and cosmetics.

---

Why Choose Chibio Biotech for E-Polylysine?

🌱 High Purity – Premium-quality E-Polylysine produced through advanced fermentation
🌎 Global Certifications – ISO22000, Kosher, Halal, FDA, EFSA, and Organic Certified
🔬 Strong R&D – Innovation-driven approach for high-performance natural preservatives
📦 Reliable Supply Chain – Guaranteed consistency and bulk supply availability
💡 Custom Solutions – Tailored formulations for different industries

---

Why Should Your Business Use E-Polylysine?

✔ Reduce dependency on artificial preservatives – Meet consumer demand for clean-label products
✔ Enhance product safety & stability – Minimize spoilage and microbial risks
✔ Ensure regulatory compliance – Approved by major food safety authorities
✔ Promote sustainability – Biodegradable and environmentally friendly alternative

---

Get in Touch – Inquiry Now!

Interested in E-Polylysine for your food, pharmaceutical, or cosmetic applications?
Contact Chibio Biotech today for high-quality supply and technical support!

📩 Email: sales@chibiotech.com
📞 Phone: +86 (0)532 66983270
🌍 Website: www.chibiotech.com

✅ Boost food safety naturally with E-Polylysine – The Future of Natural Preservation!

>More details about Comparison between Nisin & e-polylysine