What is Genipin?

Genipin is a naturally occurring, organic compound extracted from the fruit of the Gardenia jasminoides Ellis plant.

It is a highly effective, non-toxic cross-linking agent known for its versatile applications across industries such as pharmaceuticals, cosmetics, food, biomaterials, and even tattooing.

Due to its strong cross-linking properties and ability to enhance the structure and stability of proteins and other molecules, Genipin has become an increasingly popular ingredient for a wide range of health, beauty, and tattoo products.

Gardenia jasminoides Ellis genipin is an aglycone derived from an iridoid glycoside called Geniposide present in the fruit of Gardenia jasminoides Ellis.

Genipin is an excellent natural cross-linker for protein, collagen, gelatin, and chitosan cross-linking.

Plant source:
The dried and mature fruit of the Rubiaceae plant Gardenia GardeniajasminoidesEllis is the product of the hydrolysis of the geniposide in the gardenia by β-glucosidase.

CAS Number and Chemical Structure

• CAS No: 6902-77-8

Chemical Structure:

Genipin has a chemical formula of C11H14O5 and is classified as an iridoid glycoside derivative. Its structure features an ester group and an epoxide ring, which makes it highly reactive for cross-linking with proteins and other biological molecules.

Solubility of Genipin:

Genipin is soluble in organic solvents such as ethanol, acetone, and dimethyl sulfoxide (DMSO), making it easy to incorporate into various formulations.

It has moderate solubility in water, which increases at elevated temperatures, allowing for versatile application in both aqueous and non-aqueous systems.

Specification of Genipin

Benefits and Functions of Genipin

1. Natural Cross-Linking Agent: Genipin is a superior natural cross-linker, enhancing the structural integrity, stability, and durability of proteins, collagen, and biomaterials. It is widely used in producing biocompatible gels, films, and scaffolds, particularly for medical and cosmetic applications.
2. Biodegradable and Non-Toxic: Unlike synthetic cross-linkers, Genipin is non-toxic, eco-friendly, and fully biodegradable, making it a safer alternative for applications that come into direct contact with the human body.
3. Anti-Inflammatory and Antioxidant Properties: Genipin exhibits anti-inflammatory and antioxidant effects, making it beneficial in formulations for wound healing, anti-aging skincare, and dietary supplements.
4. Colorant Agent: It reacts with amino acids to produce a natural blue pigment, making it a valuable natural colorant in the food and textile industries.

Main Applications of Genipin

• Pharmaceuticals: Used for the development of biodegradable, biocompatible gels and scaffolds, Genipin promotes tissue engineering, drug delivery systems, and wound healing applications.
• Cosmetics: As a natural cross-linking agent, Genipin is an ideal ingredient for anti-aging creams, facial masks, and wound healing products, helping improve skin elasticity and hydration.
• Food Industry: Acts as a natural colorant, producing a stable blue pigment when reacting with amino acids. It’s used in coloring foods, beverages, and confectioneries.
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• Biomaterials: Commonly used to cross-link collagen, gelatin, and chitosan, Genipin is widely used in making medical sutures, tissue engineering scaffolds, and controlled drug delivery systems.
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• Tattooing: Genipin has gained popularity as a natural alternative for temporary tattoos, offering a safe, non-toxic, and biodegradable option for body art enthusiasts. When applied to the skin, Genipin reacts with amino acids, forming a long-lasting blue-black pigment, similar in appearance to traditional tattoo ink. This application has made Genipin an exciting choice for those looking for a tattoo that lasts longer than standard temporary tattoos but without the permanence of traditional ink. It’s commonly used in semi-permanent tattoo products or as an alternative to henna.

Why is Genipin Ideal for Tattoos?

1. Safe and Non-Toxic: Unlike traditional tattoo inks that may contain harmful chemicals, Genipin is derived from a natural source, making it a safer option for skin application.
2. Biodegradable and Environmentally Friendly: Genipin’s natural origin means it’s biodegradable, reducing environmental impact compared to synthetic tattoo inks.
3. Semi-Permanent Effect: The tattoos created with Genipin can last up to several weeks, providing a great option for those who want the tattoo experience without the lifetime commitment.
4. Skin-Friendly: With its anti-inflammatory properties, Genipin is gentle on the skin and less likely to cause irritation compared to other tattooing agents.

Production Flowchart of Genipin

1. Raw Material Selection: Collect the fruit of Gardenia jasminoides Ellis.
2. Extraction: Extract the raw geniposide from the fruit using water or ethanol.
3. Hydrolysis: Subject the extract to enzymatic hydrolysis to convert geniposide into Genipin.
4. Purification: Purify Genipin using column chromatography to remove impurities.
5. Concentration: Concentrate the Genipin extract.
6. Crystallization: Crystallize Genipin under controlled conditions.
7. Drying and Milling: Dry and mill the final product into a fine powder.
8. Quality Control Testing: Test for purity and compliance with specifications.

How Does Genipin Work?

Genipin functions as an effective cross-linking agent by reacting with free amino groups in proteins and collagen. This reaction forms strong covalent bonds, creating a stable and durable matrix, which enhances the mechanical strength, elasticity, and longevity of the final product. This process is particularly valuable in applications such as wound healing, tissue engineering, and anti-aging cosmetics.

Dosage Guidelines for Genipin

• Cosmetics: Use between 0.5% to 2% concentration for anti-aging and skin-firming formulations.
• Pharmaceuticals: Dosage varies based on application but typically ranges between 1% to 5% for wound healing and tissue engineering.
• Food Industry: For natural colorant purposes, use concentrations as recommended by food safety guidelines.
• For tattoo applications, Genipin is usually used in a concentration range of 2% to 5%, depending on the desired depth and intensity of the tattoo color. Its reaction with amino acids in the skin creates a deep blue or blue-black pigment, giving the tattoo a natural and realistic appearance.

It is advisable to perform compatibility testing and consult a formulator for accurate dosage based on the specific application.

Why Choose Chibio Biotech for Genipin?

• High Purity: Our Genipin boasts an assay purity of ≥98%, ensuring optimal performance and quality.
• Eco-Friendly Production: We use environmentally friendly extraction and purification processes, ensuring a sustainable product.
• Global Expertise: With years of experience in supplying bioactive compounds worldwide, Chibio Biotech has the knowledge and capabilities to support your business needs.
• Quality Assurance: Rigorous testing and quality control processes ensure that our Genipin meets the highest industry standards.
• Customizable Solutions: We provide flexible packaging and bulk orders to suit your unique requirements.

Why Use Genipin in Your Business?

• Versatile Applications: Genipin’s wide range of uses across pharmaceuticals, cosmetics, food, and biomaterials makes it a valuable ingredient for multiple industries.
• Natural and Safe: As a natural, non-toxic cross-linking agent, Genipin is a safer alternative to synthetic cross-linkers, enhancing the appeal of your products to health-conscious consumers.
• Enhances Product Quality: Improve the efficacy, stability, and durability of your formulations with the superior cross-linking properties of Genipin.
• Eco-Friendly: Using Genipin aligns with sustainable and environmentally friendly practices, making your products more attractive to eco-conscious markets.

• Why Use Genipin in Your Tattoo Products?

  • Vibrant and Long-Lasting Color: Genipin produces a rich blue-black color that lasts significantly longer than most temporary tattoo inks, providing a semi-permanent option for consumers.
  • Gentle on Skin: As a natural compound, Genipin minimizes the risk of skin irritation, making it suitable for a wider range of users, including those with sensitive skin.
  • Eco-Friendly and Sustainable: Genipin aligns with the growing demand for sustainable, non-toxic tattoo options in the market.
  • By incorporating Genipin into your tattoo products, you’ll be offering a safer, longer-lasting, and environmentally friendly alternative to traditional tattoo inks, making your brand stand out in a rapidly evolving market.

Get in Touch with Chibio Biotech Today!

Take your formulations to the next level with the natural power of Genipin. Contact us now for a customized quote or to learn more about how Genipin can transform your products. With our top-quality Genipin, you’ll be adding a highly effective, eco-friendly ingredient to your range, ensuring the success and growth of your business.